Fig. 4

TRIM67 upregulation decreases cell apoptosis in mice after cerebral ischemia‒reperfusion injury. A The expression of Bcl-xl, Bax, cleaved caspase-3, -9, and cleaved PARP in vivo was examined by western blotting. B–F Quantitative analysis of apoptosis-related chemicals is shown in A (n = 5). G TUNEL test demonstrating cell death in the MCAO/R mouse peri-infarct hippocampus and cortex. Scale bar: 50 μm. H TUNEL-positive cells after MCAO/R were counted (n = 5). The mean ± SD are displayed for all data. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001