Fig. 5

Inhibition of SETD8 expression and H4K20 methylation are associated with cisplatin sensitivity and good clinical outcome in human cervical cancer. (A) SETD8 and H4K20 methylation and γ-H2AX expression levels in pre-CT specimens of representative responder and non-responder detected by IHC staining. Images were taken at a magnification of 100× and 400× (insets). Scale bars: 10 μm. (B) SETD8 and H4K20 methylation and γ-H2AX expression levels in post-CT specimens of representative responder and non-responder. (C-J) SETD8 and H4K20 methylation level and γ-H2AX expression and drug response in specimens before (pre-CT) and after (post-CT) platinum-based chemotherapy. (K-L) Kaplan-Meier plotter analysis of cervical cancer patient groups. Patients were dichotomized by SETD8 expression level at auto select best cutoff. Error bars represent ± SD. p values were determined by Mann-Whitney U test for (C-J), (ns: not significant; *: 0.01 ≤ p < 0.05; **: 0.001 ≤ p < 0.01; ***: 0.0001 ≤ p < 0.001; ****: p < 0.0001), log-rank test for (K-L).