Fig. 4

Reduction of 53BP1 binding to DNA breaks induced by SETD8 downregulation leads to cisplatin sensitivity by inhibiting NHEJ. (A-B) Accumulation of γ-H2AX and 53BP1 following SETD8 silencing in cisplatin-treated SiHa cells for 24 h and quantification of γ-H2AX and 53BP1 foci. (C-D) Accumulation of γ-H2AX and 53BP1 following SETD8 silencing in cisplatin-treated CaSki cells for 24 h and quantification of γ-H2AX and 53BP1 foci. (E) Schematic diagram for DSB Repair Reporter. (F) NHEJ efficiency detected by DSB Repair Reporter. Cells with or without SETD8 siRNA were treated with cisplatin for 24 h. p values were determined by two-tailed Student’s t test (ns: not significant; *: 0.01 ≤ p < 0.05; **: 0.001 ≤ p < 0.01; ***: 0.0001 ≤ p < 0.001; ****: p < 0.0001)