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Fig. 3 | Cell & Bioscience

Fig. 3

From: SETD8, a frequently mutated gene in cervical cancer, enhances cisplatin sensitivity by impairing DNA repair

Fig. 3

SETD8 inhibition affects 53BP1 accumulation by reducing the methylation level of H4K20. (A) Western blot analysis of SETD8 and H4K20me1/H4K20me2 levels. Cells transfected with two different SETD8 siRNAs were treated with cisplatin for 24 h compared to siNC. (B) Immunofluorescence for H4K20me1 and 53BP1 post cisplatin in SiHa and CaSki cells, Scale bar, 10 μm. (C) Immunofluorescence for H4K20me2 and 53BP1 post cisplatin in SiHa and CaSki cells, Scale bar, 10 μm. (D-E) Plot profiles show fluorescence intensity along an oblique line quantified by ImageJ. (F-G) Pearson’s Coefficient and Overlap Coefficient calculated with JACoP by ImageJ. Pearson’s Coefficient or Overlap Coefficient > 0.5 indicates co-localization

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Cell & Bioscience

ISSN: 2045-3701

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