Fig. 3
From: Circuit- and laminar-specific regulation of medial prefrontal neurons by chronic stress
CRS markedly decreases inhibitory synaptic transmission onto dmPFC→BLA PNs in layer V. A Experimental procedures. B Schematic showing injection of AAV2/retro-hSyn-eGFP into NAc and AAV2/retro-hSyn-mCherry into BLA. C Schematic showing recording in dmPFC→BLA or dmPFC→NAc PNs. D Representative traces showing miniature excitatory postsynaptic currents (mEPSCs) in dmPFC layer II/III (scale bar: 1 s, 15 pA). E, F Summary plots of averaged mEPSC frequency (E) and amplitude (F). G Representative traces showing mIPSCs in dmPFC layer II/III (scale bar: 1 s, 20 pA). H, I Summary plots of averaged mIPSC frequency (H) and amplitude (I). J Summary plots of I/E frequency ratio in dmPFC layer II/III. K Summary plots of I/E amplitude ratio in dmPFC layer II/III. L Representative traces showing mEPSCs in dmPFC layer V (scale bar: 1 s, 15 pA). M, N Summary plots of averaged mEPSC frequency (M) and amplitude (N). O Representative traces showing mIPSCs in dmPFC layer V (scale bar: 1 s, 20 pA). P, Q Summary plots of averaged mIPSC frequency (P) and amplitude (Q). R Summary plots of I/E frequency ratio in dmPFC layer V. S Summary plots of I/E amplitude ratio dmPFC layer V