Fig. 2

BrdU staining showed cells in protrusions of central canal under syringomyelia were ependymal cells. A Foxj1, an ependymal cell marker, was positive in the protusions on SM D14 and SM D30. DAPI, blue; Foxj1, green. Scale bars: 100 μm. B Experimental plan of BrdU injection. C BrdU⁺ cells were observed in protrusions on SM D14. The right panel were magnification of the left. DAPI, blue; BrdU, green; PTB, red. Scale bars in left: 100 μm. Scale bars in right: 20 μm. D BrdU⁺ cells were observed in protrusions after decompression. The spinal tissue in SMDEC D14 group were decompressed on SM D14 followed by collection and detection after another 7 days. DAPI, blue; BrdU, green; PTB, red. The lower panels were magnifications of the upper. Scale bar in upper: 100 μm. Scale bar in lower: 30 μm