Fig. 5

miR-210-3p levels in EVs influence xenotransplanted tumor cells migration in zebrafish embryos. SK-N-AS cells were labelled with DiI dye and injected into the Yolk sac of 48 hpf Casper strain zebrafish (n = 45 per condition). Migration ability was quantified by the number of cells migrated from the site of injection to the caudal plexus. All images show xenografts anterior to the left, posterior to the right, dorsal up and ventral down. A. Schematic representation of the site of injection (yolk sac) and the site of confocal acquisition in the caudal plexus region. B, C, D. Representative confocal images (2.5X magnification) of zebrafish embryo xenografts at 24 hpi showing cells injected in the yolk sac and cells migrated to the caudal plexus region. Scale bar 200 µm. Representative confocal images (10X magnification) of migrated cells in the caudal plexus region at 24 hpi. Scale bar 100 µm E. Quantifications of migrated cells in the tail region at 24 hpi. Values are mean ± SEM