Fig. 3

miR-210-3p is highly expressed in hypoxic cells and their derived EVs. A. Scores and loading plots of the PCA model trained on the miRNA dataset (n = 6). The plot displays the first and third principal components. Color key on the loadings plot: (red) miRNAs highly expressed in hEVs; (light blue) miRNAs highly expressed in rEVs; (dark blue) miRNAs highly expressed in nEVs. B. Heatmap of the 130 miRNAs derived from SK-N-AS EVs. C. Heatmap of the 160 miRNAs derived from SK-N-DZ EVs. D. The histograms show two sets of the 10 most upregulated and 10 most downregulated miRNAs modulated in hypoxia and reoxygenation in both SK-N-AS and SK-N-DZ. E. Venn diagram highlights miR-210-3p as the common miRNA modulated in hypoxia and reoxygenation. F. Heatmap shows the fold change of miR-210-3p in normoxia and reoxygenation condition. G, H. qPCR analysis of miR-210-3p levels in SK-N-AS and SK-N-DZ cells (G) and EVs (H) cultured in normoxic, hypoxic and reoxygenation conditions. Data are represented as log2(2-ΔΔCt), miR-16-5p was used as housekeeping gene. Values are expressed as mean ± SEM