Fig. 1From: Integrated single-nucleus sequencing and spatial architecture analysis identified distinct injured-proximal tubular types in calculi rats A single-cell transcriptomic analysis of rats with calculi. A Study design and workflow of rat kidney sample processing for snRNA-seq and spatial architecture analysis. B Histochemical validation and von Kossa staining of the calcium crystals in the urolithiasis model via HE staining; the green arrows indicate the crystals. Original magnification, 10 × 40. C Uniform manifold approximation and projection (UMAP) of snRNA-seq cells recovered from the kidneys of both normal rats and rats with calculi, as well as proximal tubular and BI subsetsBack to article page