Fig. 8
From: Genetic labeling reveals spatial and cellular expression pattern of neuregulin 1 in mouse brain
Expression pattern of Nrg1 in the cerebellum. A Diagram of mouse brain sagittal section (top) and coronal section (bottom). The line in the sagittal section diagram indicates the position of the coronal section. The rectangle indicates the brain region shown in panel B. B Fluorescent images of tdTomato, Gad67-GFP and NeuN in the cerebellum of 2-month-old Nrg1-reporting mice. Scale bar, 250 μm. C Fluorescent images of tdTomato, Gad67-GFP and NeuN in three layers of cerebellar cortex from the rectangle in panel B. The arrows indicate the Nrg1-positive Purkinje cells, and the arrowheads indicate the Nrg1-expressing GABAergic interneurons. Scale bar, 50 μm. The Nrg1-reporting mice were crossed with Gad67-GFP mice to visualize the expression of Nrg1 in GABAergic interneurons. D Diagram showing the stereotaxic injection of Cre-reporting AAV into the PL of 2-month-old Nrg1Cre/+ mice. Four weeks after AAV injection, the brain slices were collected and analyzed. E Immunofluorescent images of tdTomato and PV in the PL of SC of Nrg1Cre/+ mice. The arrows indicate Purkinje cells expressing Nrg1 in adult mouse cerebellum. Scale bar, 50 μm. Sim: simple lobule; Cic: commissure of the inferior colliculus; 2cb: 2nd cerebellar lobule; GL: granular layer; PL: Purkinje layer; ML: molecular layer; PV: parvalbumin