Fig. 2
From: Interaction of TAGLN and USP1 promotes ZEB1 ubiquitination degradation in UV-induced skin photoaging
TAGLN and ZEB1 expression levels are associated with UVA-induced cellular senescence in HSFs. (a) SA-β-Gal staining assay in UV-induced HSFs. Scale bar: 20 μm. (b) Expression level changes of TAGLN, P53, MMP1, and COL1A2 after UVA irradiation as detected by Western blot. (c) Western blot images showing TAGLN expression level after TAGLN overexpression or knockdown. (d) P53, MMP1, and COL1A2 protein expression level in HSFs after TAGLN overexpression or knockdown as determined via Western blot. (e) Effect of TAGLN on the ROS content of UV-treated cells. Typical flow cytometry profiles (left) and quantitative analysis (right) of the fluorescence intensity of FITC (ROS) in HSFs. (f and g) MDA concentrations (f) and total SOD activities (g) in HSFs treated with TAGLN siRNA or UVA irradiation. MDA, malondialdehyde; SOD, superoxide dismutase. (h) ZEB1 expression level change in HSFs after UVA irradiation. (i and j) TAGLN and ZEB1 expression in HSFs at the mRNA (j) and protein (i) levels following TAGLN overexpression or knockdown as determined via qPCR and Western blot analyses, respectively. All values are expressed as mean ± SD, n.s, not significant, *P < 0.05, **P < 0.01