Fig. 4

MXC inhibits the phosphorylation of STING. a, b L929 cells (a) and U937 cells (b) were treated with 0.5 µg/ml HT‑DNA for 3 h following a 6 h of pretreatment with 500 µM MXC and the cell lysates were immunoblotted with indicated antibodies. c, d L929 cells (a) and U937 cells (b) were treated with 1 µg/ml cGAMP for 1 h following a 6 h of pretreatment with 500 µM MXC and the cell lysates were immunoblotted with indicated antibodies. e, f L929 cells (e) and U937 cells (f) were pretreated with 500 µM MXC for 6 h, 250 µM PXC for 6 h, ASA for 24 h or DS for 24 h and then treated with 0.5 µg/ml HT‑DNA for 3 h. The mRNA level of interferon-beta was analyzed by qPCR. g, h L929 cells (g) and U937 cells (h) were pretreated with 500 µM MXC for 6 h, 250 µM PXC for 6 h, 4 mM ASA for 24 h or 20 µM DS for 24 h and then treated with 0.5 µg/ml HT‑DNA for 3 h. The cell lysates were immunoblotted with indicated antibodies. i, j Representative fluorescent images of Hs27 cells stimulated with 0.5 µg/ml HT-DNA for 3 h following a 6 h of pretreatment with 500 µM MXC, indicated by IRF3 (green), p-IRF3 (red) and Hoechst (blue). Scale bar, 10 μm (i). The percentage of cells with nuclear p-IRF3 accounted from 100 views (j). Data are presented as the mean ± s.e.m. ***P < 0.001. Data represent at least three biological independent experiments