Fig. 2

MXC inhibits intracellular DNA-induced immune responses. a, b, c qPCR analysis of interferon-beta mRNA expression in BMDMs (a), human macrophages (b) and U937 cells (c) treated with 0.5 µg/ml HT-DNA for 3 h following a 6 h of pretreatment with 500 µM MXC. d, e, f qPCR analysis of interferon-beta mRNA expression in BMDMs (d), human macrophages (e) and U937 cells (f) treated with 2 µg/ml ISD for 3 h following a 6 h of pretreatment with 500 µM MXC. g, h qPCR analysis of Ifnb mRNA expression (g) and HSV-1 RNA (h) in L929 cells that were untreated or infected with HSV-1 (MOI = 5) for 4 h. i, j qPCR analysis of Ifnb mRNA expression (i) and VSV RNA (j) in L929 cells that were untreated or infected with VSV (MOI = 1) for 4 h. k, l qPCR analysis of IFNB mRNA expression (k) and HSV-1 RNA (l) in U937 cells that were untreated or infected with HSV-1 (MOI = 5) for 4 h. m, n qPCR analysis of IFNB mRNA expression (m) and VSV RNA (n) in U937 cells that were untreated or infected with VSV (MOI = 1) for 4 h. NT, untreated. ND, not detected. Data are presented as the mean ± s.e.m. **P < 0.01, ***P < 0.001. Data represent at least three biological independent experiments