Fig. 2
High plasma levels of antiCD4IgG are associated with increased TLR2 and TLR4 expression and distinct cell signaling pathway activation in B cells from PWH on ART. Total mRNA was extracted from B cells isolated from healthy controls (n = 7) and PWH on ART with high (HIV+/antiCD4high, n = 6) versus low (HIV+/antiCD4low, n = 7) anti-CD4 IgG levels. (A) Relative mRNA expression of TLR2, TLR4, TLR7, and TLR9 versus GAPDH in B cells as determined by qPCR. ANOVA tests were used to analyze data. High: antiCD4IgG > 50 ng/mL; Low: antiCD4IgG ≤ 50 ng/mL. (B) Correlations between plasma anti-CD4 autoantibody levels and TLR and MyD88 mRNA expression in B cells. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. (C) The representative immunological pathways were enriched in coherently changed genes between the two HIV groups