Fig. 2
From: RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells
H2BK120ub is deficient in the Sertoli cells in the Amh-Rnf20−/− mice. a Mass spectrometry detection of the ubiquitination of the H2B at K120 with the peptide HAVSEGTK(120)AVTK in the Rnf20Flox/Flox. The MQ software was used to analyze the data from mass spectrometry. X axis, m/z; Y axis, the intensity of ions; y, the C-terminal fragment ion (Y series). b Ubiquitinated peptide information at the position of the K120 in the Rnf20Flox/Flox. The ubiquitination modification site was not detected in the Amh-Rnf20−/−. c Immunofluorescent analysis of SOX9, H2BK120ub, and DMRT1 on serial paraffin-sections in the Rnf20Flox/Flox and the Amh-Rnf20−/− testes at 7 days after birth and adult mice. The nuclei were stained with DAPI. TRITC signals represent the localization of H2BK120ub, while FITC signals showed the localization of SOX9 or DMRT1. The white squares in the panels correspond to the enlarged panels. Sn, Sertoli cells; Sg, spermatogonia. Scale bar, 10 μm