Fig. 6

PSMD2 inhibits autophagy via ASS1-mTOR pathway. A, B The expression of indicated proteins in KYSE30 cells (A) and KYSE450 cells (B) with or without PSMD2 overexpression. C, D The p-mTOR:mTOR ratio was qualified with ImageJ software in KYSE30 cells (C) and KYSE 450 cells (D) with or without PSMD2 overexpression. N = 3. Data were presented as mean ± SD.E, F The LC3-II: LC3-I ratio was qualified with ImageJ software in KYSE30 cells (E) and KYSE 450 cells (F) with or without PSMD2 overexpression. N = 3. Data were presented as mean ± SD.G, H The expression of the indicated proteins in KYSE30 cells (G) and KYSE450 cells (H) PSMD2 knockdown in. I, J The pmTOR:mTOR total ratio was qualified with ImageJ software in KYSE30 cells (I) and KYSE 450 cells (J).K, L The LC3-II: LC3-I ratio was qualified with ImageJ software in KYSE30 cells (K) and KYSE 450 cells (L). N = 3. Data were presented as mean ± SD.M, N Western blot analysis of expression of the indicated molecules in ASS1 knockdown in KYSE30 cells (M). The pmTOR:mTOR total ratio was qualified with ImageJ software. N = 3. Data were presented as mean ± SD.O RAD001 treatment significantly repressed the proliferation of PSMD2 overexpressing KYSE450 cells implanted in mouse armpit (N = 5). Data were presented as mean ± SD, ****p < 0.0001