Fig. 6

BMP9 regulates the senescence and immunophenotype acquisition of MΦs. A SA-β-gal staining of BMDMs derived from young and aged WT mice and young and aged Bmp9.^−/− mice. B mRNA expression levels of Il1b, Il6 and Tnfa in each group. C Cytokine levels (IL-1β, IL-6, TNF-α and IL-10) in the supernatants of cells in each group as measured by ELISAs. To further study the effect of macrophage-derived BMP9 on liver injury, an F4/80-specific AAV2/8-overexpressing plasmid vector (AAV2/8-F4/80-Bmp9) was constructed. APAP-ALI (300 mg/kg) was established for 2 weeks after AAV2/8-F4/80-Bmp9 and AAV2/8-F4/80-CTRL injection. Serum and liver tissue were collected from each group of mice 24 h later. D, E Serum ALT and AST levels (D) and representative images showing H&E staining (E) of liver slices of each group. F Representative images showing TUNEL staining. G mRNA expression levels of Il1b, Il6 and Tnfa. The average target gene/Gapdh ratios of different experimental groups relative to the control group. *p < 0.05, **p < 0.01, and ***p < 0.001