Fig. 6

In vivo visualization of CG-induced inflammation and evaluation of the therapeutic efficacy of anti-inflammatory agents in living mice using N-TIP. A Representative fluorescence image showing CG-induced inflammatory lesion using N-TIP. B, C Quantification of FL signals in (A) panel. D In vivo fluorescence images showing the anti-inflammatory effects of DEX. Upon inflammation induction, DEX was administered, and then 0.4 mg/kg N-TIP was injected via the tail vein. In vivo imaging was performed at indicated times. E, F Quantification of FLs signals in (D) panel. (G and H) Measurement of paw thickness by DEX after CG (G) or PBS (H) injection. I, J Ex vivo imaging of excised organs from CG- (I) or CG + DEX- (J) injected mice. Mice were sacrificed at 5 h post-inflammation induction, and the respective organs were excised, followed by ex vivo imaging. Data are the mean ± SD of 5 mice. *p < 0.05, **p < 0.01, ***p < 0.0005, ns not significant, CF carrageenan injected foot, PF PBS-injected foot