Fig. 4

CDCA8 was identified as the downstream target of TMED3 regulating malignant melanoma. A PrimeView human gene expression array (3 v 3) was performed to identify differentially expressed genes in A375 cells with or without TMED3 knockdown. B The enrichment of the DEGs in canonical signaling pathways was analyzed by IPA. C The IPA analysis was performed to produce the TMED3-related interaction network. D, E The expression of several most significantly down-regulated DEGs was further determined via qRT-PCR (D) and western blotting (E). F The correlation between TMED3 and CDCA8 was investigated based on the samples from TCGA-SKCM. G, H Exogenous (G) and endogenous (H) Co-IP assay was used to verify whether there was protein interaction between TMED3 and CDCA8. I The protein levels of CDCA8 in melanoma and normal tissues were detected via IHC staining