Fig. 2

Crotonylation was positively correlated with HCC cell migration and invasion A Heatmap showing differentially crotonylated proteins in the MHCC-97H and MHCC-97L cell lines. B Gene ontology (GO)-based enrichment analysis of differentially crotonylated proteins. C Morphological changes in SNU449 and Huh7 cells after 25 mM sodium crotonate (NaCr) treatment. D Migration and invasion assays showing that SNU449 and Huh7 cells had higher invasive potential after 25 mM NaCr treatment. The data are presented as the means ± SD. **p < 0.001, ****p < 0.0001 (Student’s t test). E Wound healing assays showing that SNU449 and Huh7 cells exhibited greater migration capacity after 25 mM NaCr treatment. The data are presented as the means ± SD. **p < 0.01, ***p < 0.001 (Student’s t test). F Western blot analysis showing the changes in the expression of epithelial-mesenchymal transition (EMT)-related proteins after 25 mM NaCr treatment