Fig. 4

PDLIM-7 promotes the proliferation, migration, and invasion of Pca. A Silver-stained gels of immunoprecipitation proteins using anti-IgG and anti-Flag affinity beads to visualize the isolated TPM2 binding proteins. B Upper panel: list of candidate proteins from tandem affinity purification-coupled mass spectrometry. Lower panel: MS profiles of PDLIM7. C Whole-cell lysates from C4-2B and PC-3 cells immunoprecipitated with TPM2 antibody followed by Western blot analysis. D Whole-cell lysates from C4-2B and PC-3 cells immunoprecipitated with PDLIM7 antibody followed by Western blot analysis. E Double stains of TPM2 and PDLIM7. F–L PDLIM7 was overexpressed to explore its function in C4-2B and PC-3 cells. The proliferation capacities of the PCa cells were detected by CCK-8, colony formation, and EdU assays. Transwell assays were used to assess the migration and invasion ability of cells: F protein expression levels of PDLIM7 in C4-2B and PC-3 cells following PDLIM7 overexpression. G–J Cell proliferation assays: CCK-8, colony formation, and EdU assays. K, L Transwell migration and invasion assays. M Tumor growth curves of PC-3 cells express indicated protein in a xenograft mouse model. N Images of excised tumors. O Tumor weights. P Representative images of the stain of Ki67 and PDLIM7. Scale bars represent 50 μm. The data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001