Fig. 3

Low expression of TPM2 promotes androgen-independent proliferation of prostate cancer cells. A 10% CSS-FBS medium is an androgen-free medium, which was used to explore the effect of TPM2 on androgen level reactivity in C4-2B cells. Proliferation curves for C4-2B cells in CSS-FBS medium. B To study enzalutamide resistance, C4-2B cells were cultured in medium containing DMSO or 10 μM enzalutamide. Proliferation curves for C4-2B cells treated with enzalutamide. C The expression of AR-dependent genes NKX3.1 and PSA under the condition of 10% CSS-FBS medium and enzalutamide were detected by western blot in C4-2B cells. D Tumor growth curves of PC-3 cells express indicated protein in a xenograft mouse model. E Images of excised tumors after 3 weeks. F Tumor weights. G Representative images of the stain of Ki67 and TPM2. Scale bars represent 50 μm. ENZ, enzalutamide. The data were presented as mean ± SD. **P < 0.01, ***P < 0.001, ****P < 0.0001