Fig. 1

TPM2 expression was downregulated in prostate cancer. A–C Datasets GSE32571, GSE70770, and GSE71016 were included in the integrative analysis. DEGs filter criteria were fold change > 1.5 and P < 0.05. Volcano plots of DEGs in GSE32571, GSE70770, and GSE71016. D Venn diagram depicting common DEGs in GSE32571, GSE70770, and GSE71016. E KEGG pathway enrichment analysis. F PPI network of identified DEGs and yellow color indicates the hub genes. G TPM2 mRNA levels in PCa tissues were assessed from TCGA cohort. H mRNA levels in PCa samples compared to paired adjacent normal tissues. I The protein expression level of TPM2 was detected in the normal prostate epithelial cell line RWPE-1 and three human PCa cell lines. J Representative images of IHC staining of 50 normal prostate tissues and 100 PCa tissues. Scale bars represent 200 μm. K IHC score of TPM2 expression levels in normal prostate tissues and PCa tissues. The data were presented as mean ± SD. ****P < 0.0001