Fig. 6

At the non-regenerative stage 61, wild type tadpoles have reduced upregulation of genes known to be induced during tail regeneration compared to TRDKO tadpoles. A The expression of reparative myeloid genes at 0 h and 6 h after amputation in wild type tail and TRDKO tail. The expression was determined by RT-qPCR and normalized to that of rpl8. Note that all genes: mmp1 (matrix metalloproteinases 1), mmp13l, mmp25, mpo (myeloperoxidase), and mmp7, were upregulated during early stage of regeneration in both wild type and TRDKO tail after amputation but TRDKO enhanced the induction of mmp1, mmp13l, mmp13, mmp25 compared to wild type tadpoles. B TRDKO enhances the upregulation of leptin but not cyp26a1 during tail regeneration after amputation at stage 61. Notably, leptin has reported the highest significant upregulation in the 6 h vs 0 h comparison while cyp26a possesses the greatest significant decreased in the 6 h vs 0 h comparison during tail regeneration in Xenopus. C TRDKO leads to higher levels of expression of Wnt and FGF genes during the patterning and outgrowth period of tail regeneration, 72 h post-amputation at stage 61. All gene expression data were presented as mean ± SE, **P < 0.01