Fig. 5

The wild type animals lose cell proliferation in the regenerating region at 72 h post-amputation while the regenerating tail of the TRDKO tadpoles continue to have high levels of cell proliferation. A EdU (red) labeling of proliferating cells was carried out on sagittal sections of tail at 72 h post-amputation, counterstained with TUNEL (green) for apoptotic cells and Hoechst 3342 (blue) for DNA, respectively. The white dash lines indicate amputation sites and the white arrowheads point to labeled cells. Scale bar: 100 μm. B A higher magnification of area in the white dashed boxes in A. White arrow heads point to labeled proliferating cells. Scale bar: 100 μm. C Quantification of proliferating cells in the regenerated portion of the tail of wild type and TRDKO tadpoles. The EdU positive cells (red) were counted with ImageJ software and normalized against the total Hoechst positive cells (blue) and presented as mean ± SE (n = 3), **P < 0.01