Fig. 4

TRDKO tadpoles have more cell proliferation in the regenerated portion of the tail with no or fewer apoptotic cells in the uncut portion of the tail at 48 h post-amputation at stage 61 compared to the wild type animals. A EdU (red) labeling of proliferating cells was carried out on sagittal sections of tail at 48 h post-amputation, counterstained with TUNEL (green) for apoptotic cells and Hoechst 33342 (blue) for DNA, respectively. White arrow heads point to representative labeled cells. The regenerated portion of the tail is encircled with dotted lines. Scale bar: 50 μm. B A higher magnification of area in the white dashed boxes in A. White arrow heads point to representative labeled proliferating cells. Scale bar: 50 μm. C Quantification of proliferating cells in the regenerated portion of the tail (areas encircled in A) of both wild type and TRDKO tadpoles. The EdU positive cells (red) were counted with ImageJ software and normalized against the total Hoechst positive cells (blue) and presented as mean ± SE (n = 3), *P < 0.05