Fig. 3

TRDKO tadpoles have more amputation-induced apoptosis at 6 h post-amputation at stage 61. A TUNEL labeling (green) was carried out on sagittal sections of the tail at 6 h post-amputation, counterstained with Hoechst 33342 (blue), to detect apoptotic cells. Note that apoptotic cells appeared around the amputation site in both wild type and TRDKO. The white dash lines indicate amputation site. Scale bar: 300 μm. B Quantification of apoptotic cells. The TUNEL positive cells (green) were counted with ImageJ software and normalized against the total Hoechst positive cells (blue). The data are presented as mean ± SE (n = 3). **P < 0.01