Fig. 2

Wild type tadpoles at stage 61 can initiate regeneration after amputation but fails to complete the process. Sagittal sections of tail at different time points after amputation at stage 61 were stained with hematoxylin and eosin. Note that both wild type and TRDKO tail could finish wound healing, form wound epidermis and blastema at 6 h, 24 h and 48 h after amputation, respectively (A). At 72 h post-amputation, the tail bud patterning was absent in wild type tadpole, while TRDKO animal had normal regenerated structures, e.g., patterning to form notochord tip and apical ampulla (the termini of the spinal cord) (B). nc, notochord; m, muscle; sp, spinal cord; nt, notochord tip; na, neural ampulla. The black dash lines indicate amputation site. Scale bar: 300 μm