Fig. 5

RBX1 regulates PKM alternative splicing. A Western blot was performed to determine the PKM2 and RBX1 protein expression levels in ATC tissues together with the normal tissues adjacent to the tumor. B Scatter plots of RBX1 and PKM2 protein expression in ATC. C Western blot was performed to determine the PKM1 and RBX1 protein expression levels in ATC tissues together with the normal tissues adjacent to the tumor. D Scatter plots of RBX1 and PKM1 protein expression in ATC. E qRT-PCR showing expression levels of PKM1 and PKM2 in shRBX1-CAL62 cells. F Western blotting showing expression levels of PKM1 and PKM2 in shRBX1-CAL62 cells. G qRT-PCR showing expression levels of PKM1 and PKM2 in HA-RBX1 8305C cells. H Western blotting showing expression levels of PKM1 and PKM2 in HA-RBX1 8305C cells. I Compared to the tissue samples with low RBX1 expression, the PKM2/PKM1 ratio was significantly higher in those with high RBX1 expression. J, K Relative fluorescence intensities of mCherry and eGFP were quantified using ImageJ, and the fold change in the eGFP/mCherry ratio was calculated for the HA-RBX1, shRBX1, and control groups