Fig. 4

RBX1 promotes ATC progression by upregulating PKM2 expression. A, B qRT-PCR and western blotting were performed for measuring the PKM2 and RBX1 expression. C Western blotting was performed to determine the PKM2 and RBX1 expression in various groups. D Quantification of transwell assay in various groups. *P < 0.05, **P < 0.01. E Quantification and representative images of the lung metastases in various groups of nude mice (n = 6). F Cellular glucose consumption, G6P levels, ATP levels, and lactate generation in the specific groups. *P < 0.05, **P < 0.01. G, H Measurement of OCR and ECAR in the specific groups. *P < 0.05. I PKM2 and RBX1 expression levels in various groups were determined using western blotting. J. Quantification of transwell assay in diverse groups. *P < 0.05, **P < 0.01. K Quantification and representative images of the lung metastases in various groups of nude mice (n = 6). L Cellular glucose consumption, G6P levels, ATP levels, and lactate generation in the specific groups. *P < 0.05, **P < 0.01. M, N ECAR and OCR were measured in the indicated groups. *P < 0.05, **P < 0.01