Fig.7
Restoring Kv1.1 rescues neuronal excitability. A Left: example Kv1.1 current activated by stepped pulses in cKO::LGI1W183R::mCherry and cKO::LGI1W183R::Kv1.1 pyramidal neurons. Right: plots of current–voltage relationship of Kv1.1 current. B Averages of activation half-voltage, slope of activation curve, inactivation half-voltage, and slope of the inactivation curve. C Left: example APs and phase-plane plots. Right: averages of threshold, rheobase, and half-width of APs. D Left: example spikes of cKO::LGI1W183R::mCherry and cKO::LGI1W183R::Kv1.1 neurons. Right: input–output curves showing numbers of spikes as a function of injected current. E Left: example 1st and last spikes induced by a 200-pA current in cKO::LGI1W183R::mCherry and cKO::LGI1W183R::Kv1.1 neurons. Middle: half-width of 1st spike induced by different currents plotted against current. Right: ratios of the last vs 1st spike plotted against inject current. F Left: example firing showing spike-timing reliability. Right: plots of intraburst jitter as a function of recording time. G Averages of 1st ISI frequency, CV, and CV2. H Plots of fast AHP as a function of spikes. See Additional file 7: Table S5 for statistics. Grey dots indicate individual data points. *P < 0.05. **P < 0.01. ***P < 0.001