Fig.6
Reduced seizure susceptibility after Kv1.1 restoring. A AAV9-DIO-Kv1.1-mCherry or AAV9-DIO-mCherry accompanied by AAV9-DIO-LGI1W183R-GFP expressed in excitatory neurons. The viruses were injected bilaterally into the ventricles of cKO or Het mice at P0. B Representative images for quadruple-fluorescence (GFP, mCherry, CaMKII and DAPI) in the hippocampus (hip) and temporal cortex (temp lb) of cKO mice (P17). Scale bars: 1 mm (whole brain) and 50 μm (magnified). C The ratios of numbers of GFP + mCherry + vs CaMKII + cells (n = 5 mice per group) at P17-20 were 41 ± 3 (CA1; cKO::LGI1W183R::mCherry) and 44 ± 4 (CA1; cKO::LGI1W183R::Kv1.1), P = 0.64; 42 ± 3 (temp lb; cKO::LGI1W183R::mCherry) and 41 ± 7 (temp lb; cKO::LGI1W183R::Kv1.1), P = 0.88. At P35, the ratio were 41 ± 3 (CA1; cKO::LGI1W183R::mCherry) and 40 ± 5 (CA1; cKO::LGI1W183R::Kv1.1), P = 0.92; 40 ± 4 (temp lb; cKO::LGI1W183R::mCherry) and 42 ± 2 (temp lb; cKO::LGI1W183R::Kv1.1), P = 0.72. D Kaplan–Meier curves. E Reactions to PTZ injection of Het::LGI1W183R::mCherry and Het::LGI1W183R::Kv1.1 mice. Latency to generalized seizure (GS): 228 ± 31 s (Het::LGI1W183R::mCherry; n = 10) and 409 ± 59 s (Het::LGI1W183R::Kv1.1; n = 5), P = 0.033. F Example EEG recordings in cKO::LGI1W183R::mCherry and cKO::LGI1W183R::Kv1.1 mice (P35) during PTZ-induced seizures. F’ Enlarged view of EEGs in F. G Spectral analysis of EEGs. *P < 0.05