Fig.4
Downregulation of Kv1.1 activity in cKO::LGI1W183R neurons. A Schematic of whole-cell recording in cKO::LGI1WT and cKO::LGI1W183R neurons perfused with DTx-K. B Activated K+ current by stepped voltage pulses (− 70 to + 40 mV) in neurons before and after application of DTx-K (100 nM). Kv1.1 current (DTx-K-sensitive) are from current subtraction. C Kv1.1 current during the activation phase normalized to cell capacitance (current density) and plotted against command voltage. D The inactivation of Kv1.1 current by stepped voltage pulses (− 70 to + 10 mV). E Left, steady-state activation and inactivation curves of Kv1.1 current normalized to maximal conductance. Right, averages of half-voltages for the activation and inactivation curves. F Non-stationary noise analysis of Kv1.1 activation at a command voltage of + 40 mV. Current variance is plotted against the amplitude at a given time point. Single-channel conductance and number of active channels are determined by fitting a parabola to the data points. G Example APs induced by rheobase in LGI1WT and LGI1W183R neurons treated with DTx-K. H Averages of AP threshold, rheobase, half-width, and amplitude with the addition of DTx-K. I Curves of spikes vs injected current in neurons perfused with DTx-K. J Left: averages of half-width of 1st spike induced by different current injections in neurons perfused with DTx-K. Right: ratios of last vs 1st spikes plotted against injected current. K Plots of intraburst jitters as a function of recording time in neurons perfused with DTx-K. L Average values of 1st ISI frequency, CV, and CV2 in neurons perfused with DTx-K. See Additional file 5: Tables S4 for statistics. Grey dots indicate individual data points. *P < 0.05. **P < 0.01. ***P < 0.001