Fig. 5

Echocardiographic imaging can monitor nonperfect regeneration with the adhesion of X. tropicalis injured hearts. A: Representative echocardiography image of the same-age nonapical resection group under B-mode. B: Representative echocardiographic image of the injured heart at 5 daar, in which adhesion was identified at the boundary between the apical region of the regenerated heart and the surrounding tissue. C: Representative echocardiographic image of the injured heart at 10 daar, in which adhesion was identified at the boundary between the apical region of the regenerated heart and the surrounding tissue. D: Representative echocardiographic image of an injured heart at 30 daar, in which adhesion was identified at the boundary between the apical region of the regenerated heart and the surrounding tissue. E: Representative echocardiographic image of the injured heart at 45 daar, in which adhesion was identified at the boundary between the apical region of the regenerated heart and the surrounding tissue. Yellow arrow: The adhesion between the apical region of the regenerated heart and the surrounding tissue. F: Ex vivo gross observation of the regenerated X. tropicalis heart at 45 daar, in which echocardiography observation showed that the injured heart was regenerated with nonperfect regeneration with adhesion. F-f1: Representative image of the regenerated X. tropicalis heart at 45 daar after in vivo exposure. White arrow: Adhesion tissue. F-f2: Front side image of the isolated heart of F-f1. F-f3: Image of the dorsal side of the isolated heart of F-f1. F-f4: Apical side image of the isolated heart of F-f1. Black arrow: A defect after the adhesion tissue was cleaned during heart isolation. Ex vivo gross observation confirmed that the cut apex was regenerated in a nonperfect manner with adhesion, in which the boundary between the apical region of the regenerated heart and the surrounding tissue was connected to adhesion tissue. 5 daar: 5 days after apical resection