Table 1 GLIS3, NKX2.2, and PAX8 binding to nearby genes (within 5 kb regions from TSS) in comparison to their in/decreased expression in Glis3KO-LID or WT-LID thyroid
Category | geneSYM | RNA-Seq FC: LID-WT vs ND-WT | P value | RNA-Seq FC: Glis3KO-LID vs WT-LID | P value | GLIS3 target | NKX2.1 target | PAX8 target |
|---|---|---|---|---|---|---|---|---|
TH synthesis | Slc5a5 | 12.50 | 3.01E−60 | − 15.73 | 3.81E−28 | + | + | + |
Slc26a4 | 6.45 | 1.99E−29 | − 21.32 | 1.79E−14 | + | + | + | |
Tpo | 6.08 | 2.25E−64 | NS | + | + | + | ||
Tshr | 2.74 | 1.36E−08 | NS | − | + | + | ||
Iyd | 1.75 | 5.79E−09 | NS | + | + | + | ||
Slc16a2 | − 1.68 | 2.69E−05 | − 2.49 | 2.84E−33 | + | + | − | |
Duox1 | − 2.39 | 9.95E−04 | NS | − | + | + | ||
Duoxa1 | − 2.84 | 7.79E−07 | NS | − | + | + | ||
Duox2 | NS | NS | + | + | + | |||
Tg | NS | NS | + | + | + | |||
Duoxa2 | 1.72 | 2.47E−05 | NS | + | + | + | ||
TSH induced | Adm2 | 155.33 | 1.17E−140 | − 271.48 | 1.03E−267 | + | + | + |
Sod3 | 11.30 | 4.24E−90 | − 18.80 | 5.28E−113 | + | + | + | |
Cdh13 | 9.32 | 1.10E−138 | − 10.59 | 2.90E−95 | − | − | − | |
Dio1 | 3.26 | 4.44E−49 | − 2.32 | 7.68E−10 | + | + | + | |
Cdh16 | 2.57 | 5.35E−15 | − 8.17 | 4.03E−68 | + | + | + | |
Pde4d | 2.53 | 5.40E−09 | NS | − | − | − | ||
Ano1 | 2.49 | 2.84E−06 | NS | − | − | − | ||
Kcnq1 | 2.07 | 8.66E−07 | − 2.24 | 6.78E−13 | − | + | + | |
Thyroid function | Txnrd1 | 1.88 | 1.74E−12 | NS | + | + | + | |
Txnrd2 | − 1.58 | 3.07E−05 | NS | + | + | + | ||
Gnas | − 1.61 | 1.04E−06 | NS | + | + | + | ||
Fam20c | − 2.30 | 1.92E−05 | NS | + | + | + | ||
Clcn5 | NS | NS | − | − | − | |||
Dio2 | NS | NS | − | − | − | |||
Gnaq | NS | NS | + | + | + | |||
Gpx1 | NS | NS | + | + | + | |||
Kcne2 | NS | NS | − | − | − | |||
Txn1 | NS | NS | + | + | + | |||
Txn2 | NS | NS | − | − | − | |||
Thyroid TFs | Glis3 | 2.10 | 2.22E−07 | NS | + | + | + | |
Hhex | NS | NS | + | + | + | |||
Pax8 | NS | 2.03 | 2.01E−15 | + | + | + | ||
Nkx2-1 | NS | NS | + | + | + | |||
Foxe1 | NS | NS | + | + | + | |||
ECM | Adamts8 | 22.02 | 3.27E−03 | − 57.44 | 2.54E−84 | − | − | − |
Itga2 | 14.29 | 5.60E−68 | − 47.14 | 4.50E-58 | − | − | − | |
Col4a1 | 4.43 | 1.24E−34 | − 8.55 | 1.37E−91 | − | + | + | |
Cdh5 | 3.58 | 9.83E−30 | − 3.98 | 5.85E−47 | − | − | − | |
Col18a1 | 3.48 | 2.55E−19 | − 8.22 | 1.05E−105 | + | + | + | |
Col1a1 | 3.36 | 7.67E−15 | − 37.69 | 2.92E−37 | − | − | − | |
Col4a2 | 3.36 | 1.86E−25 | − 4.80 | 1.70E−44 | − | + | + | |
Col13a1 | 3.18 | 1.95E−46 | NS | − | + | − | ||
Col5a2 | 3.13 | 1.42E−21 | − 12.68 | 1.55E−78 | − | − | − | |
Col1a2 | 2.85 | 6.95E−14 | − 17.43 | 2.35E−46 | − | − | − | |
Col3a1 | 2.76 | 2.04E−13 | − 37.92 | 1.10E−53 | − | − | − | |
Col5a3 | 2.67 | 9.43E−11 | − 5.56 | 5.77E−53 | + | + | + | |
Col11a2 | 2.49 | 8.43E−17 | − 2.27 | 3.60E−06 | − | − | − | |
Col6a1 | 2.38 | 4.39E−11 | − 3.78 | 3.77E−06 | − | − | − | |
Col16a1 | 2.17 | 1.84E−14 | − 5.91 | 1.75E−60 | − | + | + | |
Col6a2 | 2.15 | 1.47E−07 | NS | − | + | + | ||
Col14a1 | 2.02 | 8.30E−10 | − 10.66 | 4.56E−34 | − | − | − | |
Col12a1 | 1.90 | 3.66E−06 | NS | + | + | + | ||
Col15a1 | 1.44 | 4.16E−03 | − 4.68 | 1.73E−47 | + | + | + | |
Itgb4 | NS | NS | + | + | + | |||
Cdh4 | NS | − 2.34 | 1.47E−18 | + | + | + | ||
Inflammation | Ccl2 | 21.36 | 4.44E−49 | − 37.84 | 7.27E−25 | − | − | − |
Ccl7 | 12.97 | 2.13E−46 | − 85.29 | 7.65E−47 | − | − | − | |
Ccl17 | 3.64 | 7.08E−04 | − 29.32 | 1.01E−14 | − | − | − | |
Ccl6 | 3.05 | 2.62E−11 | − 3.23 | 2.15E−17 | − | − | − | |
Ccl9 | 2.69 | 1.15E−10 | − 3.20 | 4.10E−15 | + | + | + | |
Ccl8 | 2.39 | 6.85E−04 | − 4.47 | 2.19E−06 | − | − | − | |
Ccl12 | 2.22 | 1.10E−03 | − 30.15 | 1.65E−18 | − | − | − | |
Il6 | 5.62 | 7.32E−07 | − 14.73 | 2.75E−10 | − | − | − |