Fig. 7
From: Mutation-class dependent signatures outweigh disease-associated processes in cystic fibrosis cells
Validation of DEPs via Western blot (WB) Ten DEPs common to all mutant cell lines were validated by WB: A G542X-CFTR, B F508del-CFTR, C N1303K-CFTR, D G551D-CFTR, and E I1234V-CFTR. The vertical axis represents the protein expression level obtained from mass spectrometry, and the horizontal axis represents the protein expression level obtained from WB. Each coloured dot represents a different protein. Correlation analysis of the same DEPs was also performed in similar mutations. Correlation analysis between protein expression obtained from western blot in each of the class I mutations F Y122X-CFTR and G W1282X-CFTR vs G542X-CFTR and each of the class II mutations H I507del-CFTR and I N1303K-CFTR vs F508del-CFTR. For a protein to be validated, the corresponding dot must fall either on the bottom left or top right square. All data are presented as mean ± SEM and relative to WT-CFTR (n = 3 biological replicates)