Fig. 3

Validation of DEGs via RT-qPCR Ten DEGs common to all mutant cell lines were validated by RT-qPCR: A G542X-CFTR, B F508del-CFTR, C N1303K-CFTR, D G551D-CFTR, and E I1234V-CFTR. The 2^−ΔΔCT method was used for data analysis using GAPDH as a housekeeping gene. The vertical axis represents the gene expression level obtained from RNA-seq, and the horizontal axis represents the gene expression level obtained from RT-qPCR. Correlation analysis of the same DEGs was also performed in similar mutations. Correlation analysis between gene expression obtained from RT-qPCR in each of the class I mutations F Y122X-CFTR and G W1282X-CFTR vs G542X-CFTR and each of the class II mutations H I507del-CFTR and I N1303K-CFTR vs F508del-CFTR. Each coloured dot represents a different gene. For a gene to be validated, the corresponding dot must fall either on the bottom left or top right square. All data are presented as mean ± SEM and relative to WT-CFTR (n = 3 biological replicates)