Fig. 1

CFTR expression in 16HBE WT-CFTR and CRISPR-engineered cell lines A CFTR mRNA expression levels determined by RT-qPCR normalized to GAPDH (housekeeping gene). B Western Blot (WB) analysis of CFTR protein expression (UNC596) and Calnexin loading control. C CFTR processing (C/C + B) was determined, and results shown normalized to WT-CFTR. Fold-change values are mean ± SEM, relative to WT-CFTR (n = 3 biological replicates). Asterisks indicate * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and ns – not significant