Fig. 6

Supplementation with L. acidophilus alleviated ethanol-induced liver injury and restored intestinal barrier function. A HE staining of liver tissues of chronic and binge ethanol-fed mice and isocaloric-fed mice with and without L. acidophilus supplementation. B Assessment of hepatic steatosis with Oil-red staining. C Serum ALT and AST levels of ALD mice with and without L. acidophilus treatment. D, E Colonic ZO-1 (D) and Occludin (E) immunofluorescence analysis of above groups. F, G In vivo evaluating gut permeability of above groups of mice by serum LPS (F) and FD4 (G) levels. (H) hepatic 16S rRNA expression determined by PCR to detect bacterial translocation of above groups. *p value < 0.05