Fig. 4

Phenylalanine supplementation worsened alcohol-induced liver injury and intestinal barrier dysfunction. A HE staining of liver tissues of alcohol-fed and isocaloric-fed mice orally treated with phenylalanine or PBS. B Evaluation of hepatic steatosis by Oil-red staining of above groups. C, D Serum ALT, AST (C) and LPS (D) levels of above mice. E Inflammatory cytokines expressions of colonic tissues measured by qPCR of above mice. F Macrophage invasion of colonic tissues measured by IHC of above mice. G Cytotoxic of primary hepatocytes treated with ethanol or phenylalanine measured by LDH release rate. H IAP expression of colonic epithelial cells with ethanol or phenylalanine measured by IF. I ZO-1 and Occludin expressions of above cells measured by western blotting. *p value < 0.05. n.s, no significant