Fig. 5
From: ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation
ANGPTL2 promotes remyelination via its receptor MAG. A Representative images of the corpus callosum region stained with oil red O in Mag+/+ and Mag−/− mice after the withdrawal of cuprizone for two additional weeks. Myelin was stained in red color. Meanwhile, more nonspecific oil red O positive deposits (a phenomenon that oil red O deposits in damaged myelin region) were observed in Angptl2−/− mice (arrows). B Quantification of the demyelinating areas in Panel A; six sections from each mouse were analyzed (n = 6). C Immunofluorescence images of MBP, MOG, MAG and GFAP staining in the corpus callosum region from Mag+/+ and Mag −/− mice after the withdrawal of cuprizone for two additional weeks. D Quantification of the fluorescence intensity of MBP, MOG, MAG and GFAP in the corpus callosum in Panel C. Four sections from each mouse were analyzed (n = 6). E Representative electron microscopy images of the corpus callosum from Mag+/+ and Mag−/− mice after the withdrawal of cuprizone for two additional weeks. Red arrow indicates uncompacted myelin lamella; F Quantification of the G ratios of the remyelinated axons of the corpus callosum in Panel E. Approximately 80 axons were counted per mouse (n = 3). G The scatter plot for the individual G-ratio values and axonal size distribution of the corpus callosum in Panel E. H Motor coordination for Mag+/+ and Mag−/− mice after cuprizone treatment for five weeks and cuprizone (Cupz) withdrawal for two additional weeks in the rotarod test; 19–22 mice were used for each group. I Representative images of the corpus callosum region stained with oil red O in Mag−/−Angptl2+/+ and Mag−/−Angptl2−/− mice after the withdrawal of cuprizone for two additional weeks. J Quantification of the demyelinating areas in Panel I; six sections from each mouse were analyzed (n = 3). K Immunofluorescence images of MBP, MOG, MAG and GFAP staining in the corpus callosum region from Mag−/−Angptl2+/+ and Mag−/−Angptl2−/− mice after the withdrawal of cuprizone for two additional weeks. L Quantification of the fluorescence intensity of MBP, MOG, MAG and GFAP in the corpus callosum in Panel K; four sections from each mouse were analyzed (n = 3). M Representative electron microscopy images of the corpus callosum from Mag−/−Angptl2+/+ and Mag−/−Angptl2−/− mice after the withdrawal of cuprizone for two additional weeks. Red arrow indicates uncompacted myelin lamella. N Quantification of the G ratios of the remyelinated axons of the corpus callosum in Panel E. Approximately 70 axons were counted per mouse (n = 3). O The scatter plot for the individual G-ratio values and axonal size distribution of the corpus callosum in Panel M. P Motor coordination in Mag−/−Angptl2+/+ and Mag−/−Angptl2−/− mice after cuprizone (Cupz) treatment for 5 weeks and withdrawal of cuprizone for two additional weeks in the rotarod test; 8–13 mice were used for each group. (*p < 0.05, **p < 0. 01, ***p < 0. 001)