Fig. 4

ANGPTL2 promotes oligodendrocyte differentiation and remyelination under pathological conditions. A Representative images of the corpus callosum region stained with oil red O in Angptl2^+/+ and Angptl2^−/− mice after the withdrawal of cuprizone for two additional weeks. Myelin was stained in red color. Meanwhile, more nonspecific oil red O positive deposits (a phenomenon that oil red O deposits in damaged myelin region) were observed in Angptl2^−/− mice (arrows). B Quantitative data of the demyelinating areas in Panel A; six sections from each mouse were analyzed (n = 7). C Immunofluorescence staining for MBP, MOG, MAG and GFAP in the corpus callosum region from Angptl2^+/+ and Angptl2^−/− mice after the withdrawal of cuprizone for two additional weeks. D Quantification of the fluorescence intensity of MBP, MOG, MAG and GFAP in the corpus callosum region in Panel C. Four sections from each mouse were analyzed (n = 6–7). E Representative electron microscopy images of the corpus callosum from Angptl2^+/+ and Angptl2^−/− mice after the withdrawal of cuprizone for two additional weeks; red arrow indicates uncompacted myelin lamella; F Quantification of the G ratios of the remyelinated axons of the corpus callosum in Panel E. Approximately 70 axons were counted per mouse (n = 3). G The scatter plot for the individual G-ratio values and axonal size distribution of the corpus callosum in Panel E. H Motor coordination of Angptl2^+/+ and Angptl2^−/− mice after cuprizone (Cupz) treatment for five weeks and cuprizone withdrawal for two additional weeks in the rotarod test; 7–8 mice were used for each group. (*p < 0.05, **p < 0. 01, ***p < 0.001)