Fig. 3
From: ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation
ANGPTL2 promotes oligodendrocyte differentiation. A Cell morphology of HCN cells after treatment with IGF1 for the differentiation to oligodendrocytes in vitro with/without ANGPTL2 at the indicated time points. B Immunofluorescence staining for MBP in HCN cells 72 h after induction in Panel A. C Quantitative data in Panel B are shown; A total of 22 or 23 sections were counted (n = 4). D Representative electron microscopy images in the optic nerve fiber and corpus callosum of the brains from Angptl2+/+ and Angptl2−/− mice on day 15; red arrow indicates the normal myelin, green arrow indicates uncompacted myelin lamella; yellow arrow indicates redundant compact myelin; purple indicates excess of cytoplasm in the periaxonal space. E Shown are the quantification data of abnormally myelinated axons including uncompacted myelin lamella, redundant compact myelin and excess of cytoplasm in the periaxonal space, in optic nerve fibers and corpus callosum. 100–410 axons in each mouse were counted (n = 3). F–I Quantification of the G ratios of the myelinated axons of the optic nerve fiber (F) or corpus callosum (H) in D. A total of 120 ~ 200 axons in each mouse were counted (n = 3). The scatter plots for the individual G-ratio values and axonal size distribution in the optic nerve fibers (G) or corpus callosum (I) in Panel D are shown. (***p < 0.001)