Fig. 2
From: ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation
Murine homologue of human MAG binds to ANGPTL2. A Flow cytometric analysis of conditioned medium with/without ANGPTL2-Flag binding to 293 T cells with transient transfection of plasmids of N1-mMAG-EGFP (murine MAG, full-length) or N1-EGFP (vector). Percentage of binding/unbinding cells to ANGPTL2 in EGFP+ cells are shown. B Quantitative data of percentage of binding cells in Panel A (n = 3). C ANGPLT2 bound to the ECD of murine MAG (murine MAG-ECD) in the conditioned medium (CM) of 293 T cotransfected cells using co-immunoprecipitation. Tie-2-ECD-FC served as a negative control. D Representative flow cytometric analysis showing that purified ANGPTL2-Flag protein enhanced GFP expression in murine MAG chimeric reporter cells (Murine MAG). Control reporter cells (control) without murine MAG-ECD were examined. E Quantitative data in Panel D (n = 3). F Binding kinetics of ANGPTL2-Flag to murine MAG-ECD were measured using a biolayer interferometry instrument (Octed RED 96/96S). (***p < 0.001)