Fig. 8
From: 14-3-3 proteins regulate cullin 7-mediated Eag1 degradation
Difopein reduces Eag1 degradation by Cul7. A The effect of R18 mutant or difopein on Eag1 degradation by Myc-tagged Cul7 (Myc-Cul7) in HEK293T cells. (Left) Representative immunoblots. Eag1 was co-expressed with increasing amounts of Cul7. (Right) Quantification of relative Eag1 protein levels with respect to the amount of Cul7 used for co-transfection. Protein densities were standardized as the ratio of Eag1 signals to the cognate GAPDH signals, followed by normalization with respect to the Myc-vector control (n = 3). B The effect of siRNA knock-down of endogenous Cul7 (siCul7) on the regulation of Eag1 protein expression by R18 mutant or difopein in HEK293T cells. (Left) Representative immunoblots. (Right) Quantification of relative Eag1 protein levels. Protein densities were standardized as the ratio of Eag1 signals to the cognate β-actin signals, followed by normalization with respect to the corresponding siRNA negative control (siControl) or R18 mutant control (*, p < 0.05; n = 3)