Fig. 11
From: 14-3-3 proteins regulate cullin 7-mediated Eag1 degradation
14-3-3 proteins contribute to Cul7-mediated degradation of disease-associated mutant Eag1 proteins. The effect of siRNA knockdown of endogenous Cul7 (A), difopein co-expression (B), or BV02 treatment (C) on WT and mutant Eag1 protein levels in HEK293T cells. (Left panels) Representative immunoblots. (Right panels) Quantification of relative Eag1 protein levels. Protein densities were standardized as the ratio of Eag1 signals to the cognate β-actin signals, followed by normalization with respect to the YFP-R18 mutant (*, P < 0.05; n = 3)