Fig. 9

Ang-(1–7) regulates microglia/macrophage polarization and inflammatory cytokine secretion through the TLR4-mediated NF-κB pathway. a Representative Western blots for iNOS, Arg-1, IL-1β, TNF-α and GAPDH expression in each group rats. b–e Bar graph showing the corresponding quantitative data. f Representative Western blots for TLR4, p-p65, p65, p-Iκb and β-actin expression in each group rats. The TLR4/ NF-κB pathway was inhibited by Ang-(1–7) treatment in the indicated group and A779 partially reversed the effects of Ang-(1–7). g–j Bar graph showing quantitative data for each group of NF-κB-related proteins. Error bars indicated the mean ± SD for three separate experiments, n = 3 for each group, ns, nonsignificant, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. one-way ANOVA followed by Tukey's Post Hoc test