Fig. 8

MasR mediates Ang-(1–7) inhibition of inflammation and improvement in promoting neurological recovery. a Double-staining for F4/80 (green)/iNOS (red) or F4/80 (green)/Arg-1 (red) in each group of macrophages (scale bar: 50 μm, n = 5). b Double-staining for Iba1 (green)/iNOS (red) or F4/80 (green)/Arg-1 (red) of spinal cord sections in each group on the 3rd day post-surgery (scale bar: 50 μm, n = 5). c–d Quantification of iNOS and Arg-1 immunofluorescence intensity in vivo and in vitro for each group of microglia/macrophages. e Representative fluorescence image of the distribution of CD68⁺ cells in the group of SCI, SCI + Ang-(1–7), SCI + Ang-(1–7) + A779 and SCI + A779 (scale bar: 500 μm, n = 3). f Quantification of CD68 immunofluorescence intensity in spinal cord tissue sections from each group. g Basso, Beattie and Bresnahan (BBB) scores. h Inclined plane test scores. Data are presented as mean ± SD; ns, nonsignificant, P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001, and**** P < 0.0001. In a–f, one-way ANOVA followed by Tukey's Post Hoc test. In g–h, two-way ANOVA followed by Bonferroni’s post hoc tests