Fig. 7

Ang-(1–7) reduced inflammatory cell infiltration and regulated inflammatory cytokines in SCI rats. a Representative fluorescence image of the distribution of CD68⁺ cells in each group of spinal cord sections (scale bar: 500 μm and 100 μm, n = 5). b–c Quantitative RT-qPCR analysis of spinal cord tissue showing mRNA expression of IL-1β, TNF-α (b), IL-10, IL-4 (c) on the 3rd day post-surgery. Data are expressed as fold change compared to the control group (n = 3). d–f Representative immunoblots and quantification showing protein expression of IL-1β (d, e) and TNF-α (d, f) in the spinal cord tissue on the 3rd day post-surgery. GAPDH: loading control. Data are expressed as fold change compared to the control group (n = 3). In a–f, data are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. One-way ANOVA followed by Tukey’s Post Hoc test