Fig. 6

Ang-(1–7) shifts the polarization of microglia/macrophages in the spinal cord toward the M2 phenotype a-b Double-staining for Iba1 (green)/iNOS (red) and Iba1 (green)/Arg-1 (red) of spinal cord tissue sections in each group on the 3rd day post-surgery (scale bar: 50 μm, n = 5). c-d quantification of spinal cord tissue sections in each group on the 3rd day post-surgery (scale bar: 50 μm, n = 5). e–g Representative immunoblots and quantification of iNOS (e, f) and Arg-1 (e, g) in the spinal cord tissue in each group on the 3rd day post-surgery. Data are expressed as fold change compared to the control group (n = 3). In a–g, data are presented as mean ± SD; *P < 0.05, **P < 0.01, and ****P < 0.0001. One-way ANOVA followed by Tukey's Post Hoc test